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["A8000艾美益试剂测试流程及方法"]
["序号","项目名称","项目缩略词","项目编号","检测范围","标本种类","样本量（uL）","缓冲液量（uL）","混合液量（uL）","参考值","建议上机量","流程及方法"]
[1,"PCT（降钙素原）","PC","02","0.1~100ng/mL","全血/血清/血浆",150,150,75,"<0.5ng/mL","全血管最少取样量1mL\n1.5子弹头最适取样量200~300uL\n0.5子弹头最适取样量200~300uL","进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取150uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[2,"hsCRP（C-反应蛋白）","CA","01","0.5~200mg/L","全血/血清/血浆",10,500,75,"hsCRP：<1.0mg/L\nCRP：<10mg/L","全血管最少取样量1mL\n1.5子弹头最适取样量50~100uL\n0.5子弹头最适取样量30~50uL","进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取10uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应3分钟→测试"]
[3,"Total βhCG（总β-人绒膜促性腺激素）","HC","07","5~50000mIU/mL","全血/血清/血浆","全血50/血清、血浆30",150,75,"未孕女性：<20mIU/mL\n绝经后女性：<20mIU/mL\n妊娠女性：\n3周 5~50\n4周 5~426\n5周 18~7,340\n6周 1,080~56,500\n7~8周 7,650~229,000\n9~12周 25,700~288,000\n13~16周 13,300~254,000\n17~24周 4,060~165,400\n25~40周 3,640~117,000","全血管最少取样量1mL\n1.5子弹头最适取样量100~150uL\n0.5子弹头最适取样量100~150uL","1.全血：进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取50uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应15分钟→测试\n2.血清/血浆：进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取30uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应15分钟→测试\n3.稀释液：取270ul稀释液到稀释试管中→取30ul样本到同一稀释试管中→吞吐混匀→取30ul（全血50ul）混合液加到缓冲液中→吞吐充分混匀→取75ul缓冲混合液添加到反应板加样孔中→反应15分钟→测试（备注：在A8000稀释操作由手动完成，不在机器完成，机器只完成稀释后的操作，但是仪器上可以做标注是稀释几倍的，不然几天后就完了）"]
[4,"Progesterone（孕酮）","PG","10","0.38~62.89ng/mL\n1.20~200mol/L","血清/血浆",30,150,75,"男性：0.45~6.55nmol/L\n女性：未怀孕-卵泡期 0.99~4.83\n      -黄体期 16.4~59.02\n-绝经<0.25~2.48\n妊娠-初期 15.04~161.35\n-中期 61.72~144.05","全血管最少取样量1mL\n1.5子弹头最适取样量100~150uL\n0.5子弹头最适取样量100~150uL","进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取30uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→25°环境下反应15分钟→测试"]
[5,"CK-MB（肌酸激酶同工酶）","CK","14","3.00~100.00ng/mL","全血/血清/血浆",75,150,75,"≤ 7.00ng/mL","全血管最少取样量1mL\n1.5子弹头最适取样量150~200uL\n0.5子弹头最适取样量150~200uL","进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取75uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[6,"D-Dimer（D-二聚体）","DD","16","50~20000ng/mL","全血/血浆",10,150,75,"≤500ng/mL","全血管最少取样量1mL\n1.5子弹头最适取样量50~100uL\n0.5子弹头最适取样量30~50uL","进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取10uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[7,"Myoglobin（肌红蛋白）","MY","15","5.00~500.00g/mL","全血/血清/血浆",10,150,75,"≤70.00ng/mL","全血管最少取样量1mL\n1.5子弹头最适取样量50~100uL\n0.5子弹头最适取样量30~50uL","进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取10uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[8,"NT-proBNP（N-端脑利钠肽前体）","NB","13","10~30000pg/mL","全血/血清/血浆",10,150,75,"≤125pg/mL","全血管最少取样量1mL\n1.5子弹头最适取样量50~100uL\n0.5子弹头最适取样量30~50uL","进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取10uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→25°环境下反应12分钟→测试"]
[9,"Tn-I plus（心肌肌钙蛋白I）","TG","12","0.01~100.00ng/mL","全血/血清/血浆",50,150,75,"≤0.04ng/mL","全血管最少取样量1mL\n1.5子弹头最适取样量100~150uL\n0.5子弹头最适取样量100~150uL","进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取50uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[10,"PCT/hsCRP（降钙素原/C-反应蛋白二联卡）","PR","22","PCT：0.05-50.0ng/mL\nCRP/hsCRP：0.5-200.0mg/L","全血/血清/血浆",35,150,75,"PCT：<0.5ng/mL\nCRP：<10.0mg/L\nhsCRP：<1.0mg/L","全血管最少取样量1mL\n1.5子弹头最适取样量100~150uL\n0.5子弹头最适取样量100~150uL","进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取35uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[11,"Tn-I/CK-MB/Myoglobin（心肌肌钙蛋白I/肌酸激酶同工酶/肌红蛋白三联卡）","CT","20","CM-MB：3.0-100.0ng/mL\nTn-I：0.01-100.0ng/mL\nMyoglobin：5.0-500.0ng/mL","全血/血清/血浆",75,150,75,"CM-MB：≤7.0ng/mL（99th percentile）\nTn-I：≤0.04ng/mL（99th percentile）\nMyoglobin：≤70.0ng/mL（97.5th percentile）","全血管最少取样量1mL\n1.5子弹头最适取样量150~200uL\n0.5子弹头最适取样量150~200uL","进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取75uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[12,"SAA（血清淀粉样蛋白A）","SA","24","5.0-300.0mg/L","全血/血清/血浆",10,1000,75,"<10.0 mg/L","全血管最少取样量1mL\n1.5子弹头最适取样量50~100uL\n0.5子弹头最适取样量30~50uL","进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取10uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应3分钟→测试"]
[13,"ST2（可溶性生长刺激表达基因2蛋白）","ST","33","2.0-400.0ng/mL","全血/血清/血浆",75,150,75,"≤35.0ng/mL","全血管最少取样量1mL\n1.5子弹头最适取样量150~200uL\n0.5子弹头最适取样量150~200uL","进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取75uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[14,"AMH（抗缪勒管激素）","AM","25","0.02-10.00ng/mL","血清/血浆",50,150,75,"女/20-24岁/1.22-11.7 ng/mL \n女/25-29岁/0.89-9.85 ng/mL \n女/30-34岁/0.576-8.13 ng/mL \n女/35-39岁/0.147-7.49 ng/mL \n女/40-44岁/0.027-5.47 ng/mL \n女/45-50岁/0.01-2.71 ng/mL \nPCOS女性/2.41-17.1 ng/mL","全血管最少取样量1mL\n1.5子弹头最适取样量100~150uL\n0.5子弹头最适取样量100~150uL","进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取50uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→35°环境下反应12分钟→测试"]
[15,"FSH (促卵泡生成激素)","FS","09","1.00-100.00 mIU/mL","血清/血浆",150,150,75,"男性： 1.27 – 19.26 mIU/mL\n女性：卵泡期 3.85 – 8.78 mIU/mL\n             排卵期 4.54 – 22.51 mIU/mL\n        黄体期 1.79 – 5.12mIU/mL\n            绝经期 16.74 – 113.59mIU/mL",null,"进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取150uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应15分钟→测试"]
[16,"LH (促黄体生成激素)","LH","08","1.00-100.00 mIU/mL","血清/血浆",150,150,75,"男性： 1.24– 8.62 mIU/mL\n女性：卵泡期 2.12– 10.89 mIU/mL\n             排卵期 19.18– 103.03 mIU/mL\n        黄体期 1.20– 12.86mIU/mL\n            绝经期 10.87– 58.64mIU/mL",null,"进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取150uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应15分钟→测试"]
[17," PRL (泌乳素)","PL","04","1.00-150.00 ng/mL","血清/血浆",75,150,75,"男性 2.64 – 13.13 ng/mL\n女性 绝经前 3.34 – 26.72 ng/mL\n        绝经期 2.74 – 19.64 ng/mL",null,"进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取75uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应10分钟→测试"]
[18,"TSH  (促甲状腺素)","TS","03","0.10-100.00μIU/mL","血清/血浆",150,75,75,"成人：0.34-5.6μIU/mL",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→混合试管扎气孔→取75uL缓冲液放入试管中→更换枪头→探测样本液面→取150uL样本加入到装有缓冲液的试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[19,"T3  (三碘甲状腺原氨酸)","T3","05","0.77-9.24nmol/L                  (0.5-6.0ng/mL)","血清/血浆",75,"  A 液： 225                    B液：75",75,"成人：1.34-2.73nmol/L",null,"进样→样本摇匀→取枪头→探测样本液面→A液小瓶扎气孔→取75uL样本加入到装有A液的试管中→吞吐充分混匀→更换枪头→探测缓冲大瓶液面高度→取75uL B液放入A液试管中→吞吐充分混匀→反应8分钟→更换枪头→取75uL混合液体加入到反应板加样孔中→25°环境下反应8分钟→测试"]
[20,"T4  (甲状腺素)","T4","06","10.23-310.00nmol/L\n(0.79-24.00μg/dL)","血清/血浆",75,"  A 液：150                    B液：75",75,"成人：78.51 – 157.01nmol/L",null,"进样→样本摇匀→取枪头→探测样本液面→A液小瓶扎气孔→取75uL样本加入到装有A液的试管中→吞吐充分混匀→更换枪头→探测缓冲大瓶液面高度→取75uL B液放入A液试管中→吞吐充分混匀→反应8分钟→更换枪头→取75uL混合液体加入到反应板加样孔中→25°环境下反应8分钟→测试"]
[21,"HbAlC (糖化血红蛋白)","HB","17","NGSP (%):4.0-15.0\nIFCC (mmol/mol):20.2-140.4\neAG (mg/dL);68.1-383.8","全血",5,"溶血剂：100 uL           缓冲液：300uL",75,"NGSP (%):4.5-6.5\nIFCC (mmol/mol):26-48\neAG (mg/dL);82.5-139.9",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→缓冲液试管扎气孔→取100uL大瓶缓冲液放入试管中→更换枪头→探测样本液面→取5uL样本加入到装有缓冲液的试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→25°环境下反应12分钟→测试"]
[22,"SAA/CRP\n(血清淀粉样蛋白A/C-反应蛋白联合)","SC","26","SAA :5.0 – 300.0mg/L\n  CRP :0.5 -200.0 mg/L","全血/血清/血浆",10,1000,75,"SAA: 10mg/L\nCRP :10mg/L",null,"进样→样本摇匀→取枪头→探测样本液面高度→缓冲液小瓶扎气孔→取10uL样本→吐到缓冲液小瓶→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应3分钟→测试"]
[23,"IL-6 (白介素-6)","IL","48","2 – 2,500 pg/mL","全血/血清/血浆",35,150,75,"< 7 pg/mL",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取35uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[24,"Pepsinogen I/II (胃蛋白酶原I/胃蛋白酶原II联合)","PS","50","胃蛋白酶原I;          2～200ng/mL\n胃蛋白酶原II;            1.5～150ng/mL","全血/血清/血浆",10,150,75,"胃蛋白酶原I;（70～165）ng/mL\n胃蛋白酶原II;（3～15）ng/mL",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取10uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应10分钟→测试"]
[25,"BNP (B型钠尿肽)","BP","55","5–5000 pg/mL","全血/血浆",35,150,75,"＜96.00 pg/mL",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取35uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→25°环境下反应12分钟→测试"]
[26,"Troponin T (心肌肌钙蛋白T)","TT","54","10.00 – 10,000.00pg/mL","全血/血清/血浆",35,150,75,"＜17 pg/mL",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取35uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[27,"Gastrin 17（胃泌素17）","GA","49","1-50pmol/L","全血/血清/血浆",50,150,75,"1-7 pmol/L",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取50uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应15分钟→测试"]
[28,"NT-proBNP/ST2（N-端脑利钠肽前体/可溶性生长刺激表达基因2蛋白联合）","待定","52","NT-proBNP:                      10-30000pg/ml;                                ST2:2.00-400.00ng/ml","全血/血清/血浆",10,150,75,"NT-proBNP:   ≤125 pg/ml;                                       ST2:≤35.0ng/ml",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取10uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→25°环境下反应12分钟→测试"]
[29,"PCT plus(超敏降钙素原）","PP","18","0.01-50.0ng/ml","全血/血清/血浆",35,150,75,"< 0.5ng/ml",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取35uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[30,"Vitamin D（25-羟基维生素D)","VD","27","5.0 – 100.0ng/mL","全血/血清/血浆",30,150,75,"缺乏：≤10 ng/ml                                 不充足： 10 < VD < 30ng/ml                          充足 ：≥30ng/ml",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取30uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→35°环境下反应12分钟→测试"]
[31,"MxA(黏病毒抗性蛋白A)","待定","36","10-300.00ng/ml","全血",35,150,75,"< 25ng/ml",null,"进样→样本摇匀→取枪头→探测缓冲大瓶液面高度→探测物质试管扎气孔→取150uL缓冲液放入探测物质试管中→更换枪头→探测样本液面→取35uL样本加入到装有反应缓冲液的探测物质试管中→吞吐充分混匀→取75uL混合液体加入到反应板加样孔中→反应12分钟→测试"]
[32,"H-FABP（心脏型脂肪酸结合蛋白）","待定","待定",null,null,null,"未开始注册先订项目名"]
[33,"Lp-PLA2（脂蛋白相关磷脂酶A2）","待定","待定",null,null,null,"未开始注册先订项目名"]